KeyTec® TR-FRET BRD4 / CRBN PROTAC Binding Assay kit
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CAT.: A1090016D
The KeyTec® TR-FRET BRD4/CRBN PROTAC Binding Assay Kit is designed for screening PROTAC molecules that mediate the formation of a ternary complex between wild-type BRD4 protein and the CRBN/DDB1 complex. This kit utilizes KeyTec® TR-FRET technology in a homogeneous immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes the Tag1-CRBN/DDB1 protein complex, while the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-BRD4 protein. In the presence of the positive control PROTAC molecule dBET1, the Tag1-CRBN/DDB1 complex and Tag2-BRD4 protein are brought into close proximity, forming a ternary complex. This allows Solar Eu (donor) and LA (acceptor) to come near each other, enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at a specific wavelength (665 nm), the PROTAC-mediated binding between BRD4 and CRBN/DDB1 can be quantitatively determined.
Standard curve
Comparison of different incubation times
| CAT. | Description | Size |
| A1090016D | KeyTec® TR-FRET BRD4 / CRBN PROTAC Binding Assay kit | 100 tests |
|
• 1 vial (8 μL/vial) Tag2-BRD4 • 1 vial (8 μL/vial) Tag1-CRBN/DDB1 • 1 vial (5 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (5 μL/vial) mAb anti-Tag2 - LA • 1 vial (5 μL/vial) TCEP • 2 vials (2 mL/vial) PROTAC Diluent Buffer #2 • 1 vial (2 mL/vial) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET BRD4/CRBN PROTAC Binding Assay Kit is designed for screening PROTAC molecules that mediate the formation of a ternary complex between wild-type BRD4 protein and the CRBN/DDB1 complex. This kit utilizes KeyTec® TR-FRET technology in a homogeneous immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes the Tag1-CRBN/DDB1 protein complex, while the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-BRD4 protein. In the presence of the positive control PROTAC molecule dBET1, the Tag1-CRBN/DDB1 complex and Tag2-BRD4 protein are brought into close proximity, forming a ternary complex. This allows Solar Eu (donor) and LA (acceptor) to come near each other, enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at a specific wavelength (665 nm), the PROTAC-mediated binding between BRD4 and CRBN/DDB1 can be quantitatively determined.
Standard curve
Comparison of different incubation times
| CAT. | Description | Size |
| A1090016S | KeyTec® TR-FRET BRD4 / CRBN PROTAC Binding Assay kit | 500 tests |
|
• 1 vial (40 μL/vial) Tag2-BRD4 • 1 vial (40 μL/vial) Tag1-CRBN/DDB1 • 1 vial (25 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (500 tests/vial) mAb anti-Tag2 - LA • 1 vial (120 μL/vial) TCEP • 1 bottle (50 mL/bottle) PROTAC Diluent Buffer #2 • 1 bottle (30 mL/bottle) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
The KeyTec® TR-FRET BRD4/CRBN PROTAC Binding Assay Kit is designed for screening PROTAC molecules that mediate the formation of a ternary complex between wild-type BRD4 protein and the CRBN/DDB1 complex. This kit utilizes KeyTec® TR-FRET technology in a homogeneous immunoassay format, offering advantages such as simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is illustrated below: The KeyTec® TR-FRET Solar Eu1-conjugated anti-Tag1 antibody specifically recognizes the Tag1-CRBN/DDB1 protein complex, while the KeyTec® TR-FRET LA2-conjugated anti-Tag2 antibody specifically recognizes Tag2-BRD4 protein. In the presence of the positive control PROTAC molecule dBET1, the Tag1-CRBN/DDB1 complex and Tag2-BRD4 protein are brought into close proximity, forming a ternary complex. This allows Solar Eu (donor) and LA (acceptor) to come near each other, enabling energy resonance transfer upon external light excitation. By measuring the signal intensity at a specific wavelength (665 nm), the PROTAC-mediated binding between BRD4 and CRBN/DDB1 can be quantitatively determined.
Standard curve
Comparison of different incubation times
| CAT. | Description | Size |
| A1090016L | KeyTec® TR-FRET BRD4 / CRBN PROTAC Binding Assay kit | 5,000 tests |
|
• 1 vial (400 μL/vial) Tag2-BRD4 • 1 vial (400 μL/vial) Tag1-CRBN/DDB1 • 1 vial (250 μL/vial) mAb anti-Tag1 - Solar Eu • 1 vial (5,000 tests/vial) mAb anti-Tag2 - LA • 1 vial (120 μL/vial) TCEP • 1 bottle (200 mL/bottle) PROTAC Diluent Buffer #2 • 1 bottle (120 mL/bottle) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
English
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